Non-fermenting Gram negative bacilli (NFGNB) are a group of bacteria which are aerobic, non sporing and most commonly they are saprophytes. They are also found as commensals in both man and animals. NFGNB are previously considered as a contaminant but now it is emerged as a important cause for life threatening nosocomial infections and also multidrug resistant organisms. Materials and methods: Six months prospective study was conducted during July 2022 to December 2022 and our aim is to characterise the non-fermenters from various clinical samples and their antibiogram. 100 isolates from various clinical samples with different age groups were included. The clinical samples like pus, urine, endotracheal aspirates, blood, sputum and body fluids were collected under aseptic precaution and identified by using standard protocol. It includes Gram’s staining, Motility Testing, Catalase test, Oxidase test, OF test and various biochemical reactions. The susceptibility testing was done by Kirby bauer disc diffusion method. Results: A total of 100 NFGNB were isolated and among that nonfermenters, Pseudomonas aeruginosa (50%) was the predominant, followed by Acinetobacter baumanii (36%), Acinetobacter lwoffi (8%), Stenotrophomonas maltophilia (4%), and Burkholderia cepacia (2%). Pseudomonas aeruginosa showed good sensitivity to Polymyxin B (100%), Meropenem and Imipenam (86%) followed by Piperacillin tazobactam (78%) and Amikacin (64%). Acinetobacter baumanii showed 100% sensitivity to Polymyxin B followed by 75% sensitivity to Meropenem and Imipenam. Conclusion: Pseudomonas aeruginosa and Acinetobacter baumanii were the commonest nonfermenters that are isolated in our study. They are associated with various infections like urinary tract infection, blood stream infections, surgical site infections and ventilator associated pneumonia. Pseudomonas aeruginosa showed better sensitivity to following antibiotics: Polymyxin B, Meropenem, Imipenem and Amikacin. Therefore it is essential to identify the nonfermenters and to know their antibiotic sensitivity pattern