Student, Department of Pharmaceutical Analysis, KLR Pharmacy College, Palwancha, Telangana 507115, India

Assistant Professor, Department of Pharmaceutical Analysis, KLR Pharmacy College, Palwancha, Telangana 507115, India
Etoposide” “and Cisplatin, RP-HPLC, Methanol ,

A new method was established for simultaneous estimation of Etoposide” “and Cisplatin by RP-HPLC method. The chromatographic conditions were successfully developed for the separation of Etoposide” “and Cisplatin by using Agilent C18 column (4.6×150mm) 5µ, flow rate was 1ml/min, mobile phase ratio was (70:30 v/v) methanol: Phosphate buffer (KH2PO4and K2HPO4) pH 3 (pH was adjusted with ortho phosphoric acid), detection wavelength was 254nm. The instrument used was WATERS HPLC Auto Sampler, Separation module 2695, photo diode array detector 996, Empowersoftware version-2. The analytical method was validated according to ICH guidelines (ICH, Q2 (R1)). The linearity study Etoposide” “and Cisplatin was found in concentration range of 50µg-250µg and 5µg-50µg and correlation coefficient (r2) was found to be 0.999 and 0.999.The precision study was precise, robust, and repeatable

14 , 1 , 2024